临床与实验病理学杂志2012，Vol.28Issue(9)：991-995,5.
甲状腺乳头状癌中BRAF基因的表达及意义
Expression of BRAF gene and its significance in papillary thyroid carcinoma
李小强 1张惠箴 1蒋智铭 1杜光烨 2陆玲娟 2董君波2
作者信息
- 1. 上海交通大学医学院附属第六人民医院病理科,200233
- 2. 上海交通大学医学院附属第三人民医院病理科,201900
- 折叠
摘要
Abstract
Purpose To investigate the correlation between BRAF mutation and the biological behavior of the papillary thyroid carcino-ma( PTC ). Methods To collected 107 cases of the formalin-fixed paraffin-embedded tissues from the patients with thyroid disease, that included 93 cases of PTC, 5 cases of thyroid follicular cancer, 1 case of medullary carcinoma and 8 cases of thyroid adenoma, and detected the BRAF mutation in the tissues by immumohistochemistry, nested-primer PCR and gene sequencing. Results In 93 cases of PTC, BRAF protein expressed in 30 cases, with positive rate of 32. 3 % , only in one case of thyroid follicular carcinoma the expression of BRAF protein was positive ( 20% ), and all negative in medullary carcinoma and thyroid adenoma. There were 30 ( 32. 3% , ) cases of BRAF mutation in 93 cases of PTC, and there were no BRAF mutation in the other type of the thyroid disease. The expression rate of BRAF protein and the BRAF mutation rate in the patients whose age was under 45 were significantly higher than whose age was over 45. And the expression rate of BRAF protein and the BRAF mutation rate of the patients whose tumor size was over 1 cm were significantly higher than whose tumor size was under 1 cm, and the difference was statistically significant ( P <0. 05 ). There was no significant relationship between BRAF protein expression, gender, lymph node metastasis, intravascular tumor thrombus, and infiltration outside of the glands, and there was no significant relationship between BRAF mutation, gender, lymph node metastasis, intravascular tumor thrombus, and infiltration outside of the glands. Conclusion BRAF mutation occurs only in PTC in this study, which may provide a new theoretical basis for the diagnosis, treatment and prognosis of the PTC.关键词
甲状腺肿瘤/乳头状癌/BRAF基因/突变Key words
thyroid neoplasms/ papillary carcinoma/ BRAF gene/ mutation分类
医药卫生引用本文复制引用
李小强,张惠箴,蒋智铭,杜光烨,陆玲娟,董君波..甲状腺乳头状癌中BRAF基因的表达及意义[J].临床与实验病理学杂志,2012,28(9):991-995,5.
