生态与农村环境学报2012，Vol.28Issue(4)：416-421,6.
一株花生根际促生菌的筛选鉴定及其特性研究
Isolation, Identification and Characteristics of a Peanut Growth-Promoting Strain of Rhizobacteria
摘要
Abstract
Seven IAA (indole-3-acetic)-producing strains were isolated from the rhizosphere of peanuts growing in red soil. Among the 7 strains, Strain L4 was the highest in IAA excreting capability (135. 67 μg ? mL-1 in 24 h of cultivation) , in addition to its phosphate-dissolving capability. Based on its morphological feature, some of its physiological and biochemical characteristics and its 16S rRNA sequence analysis, it was identified as Arthrobacter chlorophenolicus (Gen-Bank Accession No. JQ277449). Single factor tests were designed to explore conditions optimal to growth and fermentation of the strain. Results show that the optimum conditions for its growth differed from those for its IAA excretion. The conditions optimal both to its growth and to its IAA secretion were 5 - 6 in initial pH, volume 50 mL ? (250 mL)-1 in liquid volume in flask, 30 ℃ in temperature, and 24 h of shaking culture. The best carbon and nitrogen sources for its growth was maltose and yeast extract, but for its IAA production, xylose and KNO3, respectively.关键词
红壤/根际促生菌/吲哚乙酸(IAA)/条件优化Key words
red soil/ PGPR/ indole-3 -acetic acid (IAA) / optimization分类
农业科技引用本文复制引用
李引,虞丽,李辉信,徐莉,焦加国,胡锋..一株花生根际促生菌的筛选鉴定及其特性研究[J].生态与农村环境学报,2012,28(4):416-421,6.基金项目
国家科技支撑计划(2009BADC6B003) （2009BADC6B003）
