南京林业大学学报（自然科学版）2012，Vol.36Issue(4)：84-88,5.
黑曲霉外切葡聚糖酶基因密码子的优化及表达
Codon optimization of cellobiohydrolase gene of Aspregillus niger and its expression in Pichia pastoris
摘要
Abstract
NCBI analysis revealed that there were sixteen active sites in cellobiohydrolase gene (cbhl) from Aspregillus niger, and were found rare codons around six active sites. To improve expression efficiency of recombinant cellobiohydrolase in Pichia pastoris KM71H, those rare codons around active site of cbhl were replaced with preferable codons used in P. pastoris by long-distance inverse PCR. The recombinant plasmid pPICZaA -cbhlm which contained the modified cbhl sequence was transformed in P. pastoris KM71H. After methanol induction, the expression level of cbh\m was 17 % higher than that of cbhl.关键词
外切葡聚糖酶/定点突变/密码子优化/黑曲霉/毕赤酵母Key words
cellobiohydrolase/ site-directed mutagenesis/ codon optimization/ Aspregillus niger/ Pichia pastoris分类
生物科学引用本文复制引用
李国庆,范松,裴建军,赵林果,高树峰..黑曲霉外切葡聚糖酶基因密码子的优化及表达[J].南京林业大学学报（自然科学版）,2012,36(4):84-88,5.基金项目
国家自然科学基金项目(31070515) （31070515）
江苏高校优势学科建设工程资助项目 （）
