南京农业大学学报2012，Vol.35Issue(4)：8-14,7.
转胱硫醚-γ-合酶基因高蛋氨酸大豆对土壤细菌群落结构的影响
Effects of high producing methionine soybean transferred cystathionine γ-synthase gene on community structure of bacteria in soil
摘要
Abstract
PCR-DGGE of 16S rDNA V3 region was used to research the effects of high producing methionine soybean transferred cys-tathionine y-synthase gene on community structure of bacteria in soil. The results demonstrated that, compared with non-genetically modified soybean receptor,high producing methionine soybean transferred cystathionine γ-synthase gene inhibited the growth of some bacteria belonging to phylum Acidobacteria, Gemmatimonadetes, and stimulated the growth of others bacteria belonging to phylum Proteobacteria,Cyanobacteria. Thus,it changed the community structure of bacteria in soil. Time was the main factor which affected the community structure of soil bacteria,and the study of bacterial diversity in soil revealed that bacterial diversity was lowered in some degree. But the influence was weakened gradually with the growth of soybean,and disappeared when the soybean matured.关键词
转基因大豆/DGGE/土壤细菌/群落结构Key words
transgenic soybean/DGGE/soil bacteria/community structure分类
生物科学引用本文复制引用
浦传亮,梁晋刚,高金玉,吴存祥,张明荣,张正光,崔中利,曹慧..转胱硫醚-γ-合酶基因高蛋氨酸大豆对土壤细菌群落结构的影响[J].南京农业大学学报,2012,35(4):8-14,7.基金项目
国家转基因生物新品种培育重大专项(2008ZX08011-003) （2008ZX08011-003）
