南京农业大学学报2012，Vol.35Issue(4)：41-46,6.
十字花科植物HSP22基因同源序列的克隆与进化分析
Cloning and evolutionary analysis of homologous sequences of HSP22 gene in Cruciferae
摘要
Abstract
HSP22 gene analogues from Brassica rapa subsp. pekinensis, Brassica chinensis t Raphanus sativus L. , Brassica rape f Brassica oleracea L. var. botrytis L. ,Capsella bursa-pastoris L. , Brassica juncea Coss and Brassica napus, 10 species of 3 genera in Cruciferae were obtained by PCR strategy using gene specific primers designed based on the full-length sequence of previously reported AtHSP22 gene and other similar sequences in homologous species. The secondary structure and three-dimensional structure were also predicted. Sequences alignment indicated that the similarities of the genes were more than 89-3% and 92. 1% at nueleotide and amino acid levels,respectively. The NJ( Neighbor-joining)evolutionary tree showed that Brassica was closely related to Capsella, followed by Raphanus and Arabidopsis. The senior structures predicted by amino acid sequences displayed marked differences,the most content was random coil,and α-helix all reached to 30% ,so it belongs to α. structural proteins.关键词
十字花科植物/HSP22基因/克隆/进化分析Key words
Cruciferae /HSP22 gene/ clone evolutionary analysis分类
农业科技引用本文复制引用
程影,黄锐之,钟新民,侯喜林,吴学龙,王五宏,李必元,刘智宏..十字花科植物HSP22基因同源序列的克隆与进化分析[J].南京农业大学学报,2012,35(4):41-46,6.基金项目
国家863计划项目(2008AA02Z103) （2008AA02Z103）
浙江省农业科学院科技创新项目 （）
