南京医科大学学报（自然科学版）2012，Vol.32Issue(6)：763-767,5.
低表达核糖体蛋白L22对人肺动脉平滑肌细胞增殖的影响
The effects of siRNA against RPL22 on the proliferation of human pulmonary arterial smooth muscle cells
摘要
Abstract
Objective:To investigate the effects of small interference RNA(siRNA) against ribosomal protein L22 (RPL22) on ET-1 induced proliferation of human pulmonary arterial smooth muscle cells (HPASMC). Methods: The experimental models of primary cultured HPASMC induced by ET-l were established in vitro. HPASMC were transfected with siRNA-RPL22,incubated in smooth muscle cell culture medium(SMCM). The mRNA and protein expresson levels of RPL22 in HPASMC were measured by real-time PCR and Western blot, respectively. The HPASMC proliferation was evaluated by PCNA expression and flow cytometry analysis. Results:Compared with control groups,RPL22 expression in HPASMC was significantly inhibited after siRNA-RPL22 transfection (P < 0.05),and HPASMC proliferation was significantly inhibited after siRNA-RPL22 transfection. Conclusion;The data suggest that interruption of RPL22 expression can inhibit HPASMC proliferation. It's a novel finding needs to be further explored.关键词
肺动脉平滑肌细胞/核糖体蛋白L22/增殖/肺动脉高压Key words
pulmonary arterial smooth muscle cell/ribosomal protein L22/proliferation/pulmonary arterial hypertension分类
医药卫生引用本文复制引用
孙凯,薛鸿,解卫平,王虹..低表达核糖体蛋白L22对人肺动脉平滑肌细胞增殖的影响[J].南京医科大学学报（自然科学版）,2012,32(6):763-767,5.基金项目
国家自然科学基金资助(30971319) （30971319）
