南京医科大学学报（自然科学版）2012，Vol.32Issue(6)：795-799,5.
microRNA-335对人非小细胞肺癌细胞迁移、侵袭及增殖能力的影响
Effect of microRNA-335 on migration,invasion and proliferation of NSCLC cell
摘要
Abstract
Objective:To explore the expression of microRNA-335(miR-335) in non-small cell lung cancer(NSCLC) and its effect on migration, invasion and proliferation of NSCLC cell. Methods: Real-time quantitative PCR was used to detecte and compare the expression of microRNA-335 in 12 pairs of non-small cell lung cancer.adjacent normal tissue,NSCLC cell line SPCA-l and normal epithelium cell line 16HBE. The expression of miR-335 in SPCA-l cell was down-regulated by anti-miR-335 transient transfection with Lipofectamine 2000,and the effect was identified by real-time quantitative PCR. Cell migration and invasion was measured in vitro by wound healing assay and transwell assay. The influence of miR-335 on SPCA-l cell proliferation was detected by MTT rassay and colony formation assay. Results:Compared with adjacent normal tissues,miR-335 in non-small cell lung cancer was significantly up-regulated(P < 0.05). Smilar to thai,the expression of miR-335 was significantly increased in SPCA-l cell compared to 16HBE cell (P < 0.05). The miR-335 expression of SPCA-l cell was significantly down-regulated 24 hours after anti-miR-335 transfection (P < 0.01). In SPCA-l cell,decreased miR-335 significantly inhibited cell migration and invasion,with the inhibiting rates of (42.8 ± 2.7)%(P < 0.01) and (73.25 ± 4.4)% (P < 0.01),respectively. But,no effect on SPCA-l cell proliferation was observed when the expession of miR-335 was inhibited. Conclusion;The expression of miR-335 is up-regulated in NSCLC, Down-regulaling expression of miR-335 significantly inhibites migration and invasion of SPCA-l cell while has no effct on cell proliferation.关键词
microRNA-335/非小细胞肺癌/迁移/侵袭/增殖Key words
microRNA-335/non-small cell lung cancer/migration/invasion/proliferation分类
医药卫生引用本文复制引用
王鹤,刘志利,德伟,王朝霞..microRNA-335对人非小细胞肺癌细胞迁移、侵袭及增殖能力的影响[J].南京医科大学学报（自然科学版）,2012,32(6):795-799,5.基金项目
国家自然科学基金(30973477) （30973477）
江苏省自然科学基金(BK2010590) （BK2010590）
江苏省六大人才高峰项目(09-B1-021)资助 （09-B1-021）
