南京医科大学学报（自然科学版）2012，Vol.32Issue(7)：903-907,5.
大鼠脑缺血再灌注后海马内Nemo样激酶的表达
The expression of Nemo-like kinase in hippocampus of rat following cerebral ischemia
摘要
Abstract
Objective;To investigate the expression and cellular localization of Nemo-like kinase (NLK) in hippocampus of rat following cerebral ischemia reperfusion (IR). Methods:Sixty adult male Sprague-Dawley rats were randomly assigned to two groups; sham-operated group and IR group. According to different observation time after surgery, IR rats were individed into six subgroups (1 h,4 h,8 h,l d,3 d and 7 d groups). A middle cerebral artery occlusion (MCAO) model in adult rats was constructed. The expression changes of NLK and active Caspase-3 in hippocampus of rat following cerebral IR were detected by Western blot. The cellular location and possible biological behavior of NLK was investigated by immunohistochemistry and immunofluorescence staining. Results;Western blot result revealed that NLK expression gradually increased,reached a peak at 8 h after cerebral IR,then decreased, and finally increased. There were significant differences in NLK expression between sham-operated group and IR group at 8 h after reperfusion(P < 0.05). Moreover,Caspase-3 expression progressively increased and reached a peak at the 3rd day,then decreased. There were significant differences in caspase-3 expression between these two groups at the 3rd day after reperfusion (P < 0.05). Immunofluorescence staining showed that NLK expression was principally located in neurons,especially pyramidal neurons of CA1 area in the hippocampus. Conclusion;The expression of NLK remarkably increased after cerebral IR,suggesting that NLK was involved in the development of cerebral ischemia in adult rats.关键词
脑缺血再灌注/Nemo样激酶/海马Key words
cerebral ischemia reperfusion/Nemo-like kinase/hippocampus分类
医药卫生引用本文复制引用
崔晓利,刘正霞,陈燕萍,沈玉洁,鲁翔..大鼠脑缺血再灌注后海马内Nemo样激酶的表达[J].南京医科大学学报（自然科学版）,2012,32(7):903-907,5.基金项目
国家自然科学基金(81070275) （81070275）
