南京医科大学学报（自然科学版）2012，Vol.32Issue(7)：928-932,5.
Graves病母鼠分娩后甲状腺自身抗体水平的观察
Autoantibodies in post-partum mice with Graves' disease
摘要
Abstract
Objective;To illustrate the influence of "autoantibodies" in a post-partum period of a Graves' disease animal model. Methods:Female Balb/c mice aged 6 weeks were randomly divided into experimental group and control group,and injected intramuscularly with Ad-TSHR-289 (3.3 × 108 pfu/50 μl PBS) and control adenovirus (Ad-β-gal 3.3 × 108 pfu/50 μl PBS) respectively, three times at 3 weeks intervals.When the immunization was over, both groups were mated with male mice after getting a bit of serum from venae angularis for measurement of TRAb liter and T4 level. The mice were sacrificed 1 day after delivery, blood and thyroids were obtained. T4 level was detected by radio immunoassay. Levels of TRAb, TPOAb,TGAb were detected by electrochemiluminescence immunoassay. The thyroids were used for histological examination. Resulls:TRAb positive rates were 96.7% in experiment group (P < 0.05). The incidence of hyperthyroidism in mice after delivery was 73.3%,which was higher than that after the 3rd immunization (56.7%). TPOAb positive rates were 16.7% in experiment group. The titre of TGAb in experiment group increased siginificantly higher than that in control group (P < 0.05). Conclusion:An animal model of Graves'disease is successfully prepared by immunization with Ad-TSHR-289. The TRAb maybe involved in the increase of TPOAb and TGAb in post-partum mice with Graves' disease.关键词
Graves病/甲状腺自身抗体/动物模型/分娩后Key words
Graves' disease/ autoantibodies/ animal model/ post-partum分类
医药卫生引用本文复制引用
余月,王曙,石星,朱子阳,王旭,郭梅,刘倩琦..Graves病母鼠分娩后甲状腺自身抗体水平的观察[J].南京医科大学学报（自然科学版）,2012,32(7):928-932,5.基金项目
江苏省高校自然科学研究计划第二批项目(07KJD320144) （07KJD320144）
