南京医科大学学报（自然科学版）2012，Vol.32Issue(7)：948-951,4.
血小板环氧化酶及P-选择素的检测及其在冠心病患者中的表达
Determination of platelet cyclooxygenases and P-selectin in patients with coronary artery disease
摘要
Abstract
Objective;To establish a method of delecting cyclooxygenase (COX)-l ,COX-2 and P-selectin in platelet-rich plasma (PRP) by flow cytometry (FCM),and to detect the expressions of COX-l.COX-2 and P-selectin in PRP from coronary artery- disease (CAD) patients without using anti-platelet agents. Methods;Twenty CAD patients were recruited,who were going to receive coronary artery bypass graft(CABG) and had withdrawn aspirin and clopidogrel for at least 7 days. After overnight fasting,the patients' venous blood was drawn and processed,then the PRP was incubated with fluorescence labeled antibody,and C0X-l,C0X-2 and P-selectin were detected by FCM. Results:The positive percent of COX-1 ,C0X-2 in PRP of the enrolled 20 patients were (39.67 ± 9.20)% and (4.11 ± 2.74)%,respectively,and the positive percent of P-selectin in platelet was(13.56 ± 8.07)% after incubated with saline and (24.08 ± 13.49)% after incubated with arachidonic acid,the real positive percent of P-selectin in platelet was (10.27 ± 7.40)%. Conclusion;FCM can effectively detect the platelet expressions of COX-1 ,COX-2 and P-selectin. There would be ethnic differences between Chinese Han and Caucasian regarding the platelet expressions of COX-1 .COX-2 and P-selectin in CAD patients.关键词
环氧化酶-1/环氧化酶-2/P-选择素/流式细胞术/冠心病Key words
cyclooxygenase-1 /cyclooxygenase-2/P-selectin/flow cytometry/coronary artery disease分类
医药卫生引用本文复制引用
龚晓璇,张锦英,张秋,朱甜甜,王晓伟,王学忠,殷咏梅,李春坚,杨志健..血小板环氧化酶及P-选择素的检测及其在冠心病患者中的表达[J].南京医科大学学报（自然科学版）,2012,32(7):948-951,4.基金项目
国家自然科学基金(81170181) （81170181）
江苏省人民医院创新团队基金(2011) （2011）
