南京医科大学学报（自然科学版）2012，Vol.32Issue(7)：1029-1032,4.
血清差异表达多肽谱用于人卵巢癌诊断的研究
Mass spectrometry analysis of sera peptides in diagnosis of ovarian cancer
摘要
Abstract
Objective:To establish the expression profiles of peptides in sera of ovarian cancer and explore the early-diagnosis value of expression profiles of peptides in ovarian cancer. Methods:Magnetic binding-weak cation exchange beads (MB-WCX) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis were used to detect expression files of peptides. The early-diagnosis pattern was established by differential peptides peaks using the ClinProTool data analysis program. Results: In the mass range from 1 000 to 15 000 Da,8 peaks with clear differences in amplitude between the patients and health controls were detected,5 peaks with mass charge ratio (m/z) of 2 662.02, 2 653.68,5 859.21,5 901.69 and 5 937.06 were up-regulated notably,and 3 peaks with m/z of 1 857.82,2 074.28 and 2 856.33 were markedly down-regulated in patients with ovarian cancer. In specimens of early-stage ovarian cancer,the accuracy of characteristic proteomic patterns detection could be 81.8% (9/11),and the specificity showed 100%. Conclusion;MB-WCX separation coupled with MALDI-TOF-MS and ClinProTools analysis could be a desired method to find bio-markers in ovarian cancer,and could have a potential value in the early diagnosis of ovarian cancer.关键词
卵巢癌/磁珠富集/时间飞行质谱/血清多肽Key words
ovarian cancer/magnetic binding/matrix-assisted laser desorption/ionization lime-of-flight mass spectrometry/ serum peptides分类
医药卫生引用本文复制引用
仇志琴,黄玉政,陶永辉,张英,虞丰..血清差异表达多肽谱用于人卵巢癌诊断的研究[J].南京医科大学学报（自然科学版）,2012,32(7):1029-1032,4.基金项目
江苏省自然科学基金(BK2011164) （BK2011164）
江苏省卫生厅科研项目(X201110) （X201110）
