南京医科大学学报（自然科学版）2012，Vol.32Issue(7)：1033-1037,5.
糖尿病肾病差异蛋白质组分析
Proteome analysis of diabetic nephropathy urinary
洪侃 1郁志明 2石维 3刘志华 2许宇静 1卞坤娟 2黄玉政 2徐明4
作者信息
- 1. 苏州大学附属第一医院心内科,江苏苏州215006
- 2. 南京医科大学附属无锡人民医院内分泌科,江苏无锡214023
- 3. 南京医科大学附属无锡人民医院心内科,江苏无锡214023
- 4. 江苏省血吸虫病防治研究所,江苏无锡214064
- 折叠
摘要
Abstract
Objective:To detect urinary polypeptide patterns in stages of the type 2 diabetic nephropathy (DN) in order to screen and find out the potential biomarkers. Methods: A total of 68 urine samples from type 2 diabetic patients with normoalbuminuria (DM1,n = 24),microalbuminuria (DM2,n = 22)and macroalbuminuria (DM3,n = 22),and healthy controls (C,n = 20) were condensed by magnetic beads based weak cation exchange chromatography (MB-WCX),and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MAID1-TOF-MS). ClinProtTM software was used to profile and screen the polypeptide patterns in urine of the patients. Results; When the relative molecular mass < 12 000,there were significant differences in 15 protein peaks between group DM1 and group C(P < 0.01). There was one protein peak of significantly low expression in the group DM2,when compared to group C(P= 0.029 8). And there were significant differences in 10 protein peaks between group DM3 and group C(P < 0.01). Conclusion:MB-WCX coupled to MALDI-TOF-MS is a fast,convenient and high throughput analyzing method capable of screening some relatively specific and potential biomarkers from the urine of DN with different stages.关键词
磁珠分离/基质辅助激光解吸电离-飞行时间质谱/糖尿病肾病/尿液/多肽Key words
magnetic beads separation/matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/diabetic nephropalhy/ urine/ polypeptide分类
医药卫生引用本文复制引用
洪侃,郁志明,石维,刘志华,许宇静,卞坤娟,黄玉政,徐明..糖尿病肾病差异蛋白质组分析[J].南京医科大学学报（自然科学版）,2012,32(7):1033-1037,5.
