农业环境科学学报2012，Vol.31Issue(7)：1404-1411,8.
惠州地区小型村镇水源产毒蓝藻污染初探
Analysis of Microcystin-producing Cyanobacterial in Small Rural Surface Water Samples from Huizhou,China
摘要
Abstract
Microcystins(MCs) are potent hepatotoxins produced by microcystin-producing cyanobacteria and give a significant health hazard to human and animals. MCs are normally produced by many, but not all strains of the cyanobacteria genera Planktothrix, Mcrocystis, Anabae-na. To investigate the microcystin-producing cyanobacteria in rural surface water samples, different types of rural surface water were sampled from Huizhou, including pool water, reservoir water and running water. Molecular techniques and HPLC were employed to detect the microcystin-producing cyanobacteria and MCs. The results showed that mcyA-Cd, the specific fragment coding for the microcystin synthetase complex, was detected in 12 out of 27 samples. PCR-DGGE in combination with cloning and sequencing proved that the primary detection of microcystin-producing cyanobacteria in the environmental sample using mcyA-Cd was of high specificity and accuracy. HPLC analysis indicated that MC-RR or MC-LR was detected in 8 samples, and exceeded 1 μg ·L‐1 in 5 samples out of them. These results suggest that mcyA-Cd could be applied to the rapid and accurate detection of microcystin-producing cyanobacteria in environmental samples, and that the water resources of the sampling sites had been contaminated by microcystin-producing cyanobacteria. Appropriate measures should be taken to monitor the microcystin-producing cyanobacteria in the rural surface water in Huizhou, in order to avoid or reduce the potential hazards to human.关键词
产毒蓝藻/微囊藻毒素/PCR-DGGE/HPLCKey words
microcystin-producing cyanobacteria/ microcystins/ PCR-DGGE/ HPLC分类
资源环境引用本文复制引用
袁志勇,朱红惠,霍光华,冯广达,陈远良..惠州地区小型村镇水源产毒蓝藻污染初探[J].农业环境科学学报,2012,31(7):1404-1411,8.基金项目
国家科技重大专项“水体污染控制与治理”资助项目(2008ZX07425-002) （2008ZX07425-002）
