农业环境科学学报2012，Vol.31Issue(7)：1431-1437,7.
微生物燃料电池阳极生物膜微生物群落的PCR-DGGE分析
Microbial Community of Anodic Biofilm of Microbial Fuel Cells Revealed by PCR-DGGE Analysis
摘要
Abstract
Two microbial fuel cells(MFCs) equipped with ethylenediamine and ferric trichloride modified anodes were operated continuously by using swine manure marsh-gas tank sludge as the inoculums. The MFCs could generate electricity from wastewater, and the pollutants could also be efficiently removed as well. In order to better understand microbial community of the anodic biofilm of the MFCs, the anodic biofilm samples of the MFCs including start-up phase(S), electricity-producing stationary phase(RG), and real wastewater electricity-producing stationary phase (RS) were collected and analyzed by using PCR-DGGE method. The results showed that the microbial diversity appeared significant differences among the different anodic biofilm samples. The microbial community similarity of S-RG, S-RS and RG-RS was 70.1 %, 42.0 % and 50.6 %, respectively. Besides, the microbial community similarity of the anodic biofilm of the two MFCs when fed with raw wastewater was only 48.0 %, which implied that the anodes modified with the two different methods had a selective effect on microbes. The sequencing and comparison of the DGGE bands revealed that the dominant bacteria of the anodic biofilm were Trichococcus sp., Thauera sp., Azowcus sp., Azospirillum sp., Zobelklla sp., Pseudomonw sp., Aeromonas sp., Thiobacillus sp., Desulfovibrio sp., and Thiomonas sp., respectively. Among them, Pseudomonas sp.,Aeromonas sp. and Desulfovibrio sp. were the possible high electrochemically active bacteria in this study. It was indicated that the microbial community structures were significantly different among the different biofilm samples and the anodes modified by different methods. The results provide an academic basis for understanding the development of anodic biofilm of MFCs.关键词
微生物燃料电池/养殖废水/改性阳极/PCR-DGGE/微生物群落结构Key words
microbial fuel cell/ swine wastewater/ modified anode/ PCR-DGGE/ microbial community structure分类
资源环境引用本文复制引用
王华金,朱能武,李冲,程丹,吴平霄..微生物燃料电池阳极生物膜微生物群落的PCR-DGGE分析[J].农业环境科学学报,2012,31(7):1431-1437,7.基金项目
国家自然科学基金项目(30800796) （30800796）
教育部新世纪优秀人才支持计划项目(NCET-11-0166) （NCET-11-0166）
教育部高校博士点专项基金项目(20070561082) （20070561082）
广东省科技计划项目(2007A020100001) （2007A020100001）
