农业环境科学学报2012，Vol.31Issue(8)：1461-1466,6.
O3污染胁迫下冬小麦的伤害症状及其对叶片氮代谢脯氨酸和谷胱甘肽含量的影响
Effects of Ozone Stress on Visible Injury Symptom, Nitrogen Metabolism, and the Contents of Proline and Glutathione in Winter Wheat Leaves
摘要
Abstract
Effects of O3 stress on visible injury symptom, nitrogen metabolism, and the contents of proline and glutathione of winter wheat leaves were studied by open top chambers(OTC). The results showed that O3 stress could induce significant damage to winter wheat leaves and the injury symptom was more serious under higher concentration of O3. The activities of nitrate reductase in leaves of winter wheat reduced with increasing the concentration of O3. Compared to the control treatment, at the O3 concentrations of 40-120 nL·L‐1, the activities of nitrate reductase decreased respectively by 45.4%-92.9%, 58.0%~93.5% and 90.0%~93.2% in the jointing stage, the heading stage and the milk stage of winter wheat. O3 stress also had significant effects on the contents of NH4+-N and NO3‐-N in winter wheat leaves, but the effects were different under different O3 concentrations or different growth stages. The contents of proline and glutathione varied under different O3 concentrations. Compared to the control treatment, proline contents in leaves of winter wheat in the heading stage increased respectively by 163.9% and 173.2% at the O3 concentrations of 40 nL·L‐1 and 80 nL·L‐1, while decreased by 42.4% at the O3 concentration of 120 nL·L‐1. O3 stress resulted in increase of GSH contents in leaves of winter wheat in the jointing stage, while GSH contents in leaves of winter wheat in the heading stage decreased significantly compared with the control group under elevated O3. This study suggests that plants show different adjustment and adaptation mechanism under O3 stress.关键词
臭氧/冬小麦/氮代谢/硝酸还原酶/脯氨酸/谷胱甘肽Key words
ozone/ winter wheat/ nitrogen metabolism/ nitrate reductase/ praline/ glutathione分类
农业科技引用本文复制引用
黄益宗,钟敏,隋立华,王玮,耿春梅,殷宝辉..O3污染胁迫下冬小麦的伤害症状及其对叶片氮代谢脯氨酸和谷胱甘肽含量的影响[J].农业环境科学学报,2012,31(8):1461-1466,6.基金项目
国家自然科学基金面上项目(41071336) （41071336）
国家环保公益性行业科研专项(200809152) （200809152）
