农业环境科学学报2012，Vol.31Issue(8)：1537-1543,7.
两株PGPR对豇豆苗期生长及土著细菌群落的影响
Effects of Two Plant Growth-promoting Rhizobacteria ( PGPR ) on Yardlong Bean Early Seedlings Growth and Indigenous Soil Bacterial Community
摘要
Abstract
The object of the present paper is to explore the effect of two PGPRs, Pseudomonas chlororaphis RA6 and Bacillus pumilus WP8, on yardlong bean growth, and the difference of two methods of application, seeds soaking and soil drenching, as well as PGPR survival and its impact on the indigenous bacterial community. The results showed that PGPR effects of isolated strains on yardlong bean growth depended on inoculation methods and inoculation size. By seed soaking with WP8 and RA6, germination rate of yardlong bean seeds was increased by 14.29% and 9.52% respectively(P<0.05) from the control (CK); plant height at 15 days after germination was increased by 14.39% and 10.40%(P<0.05 ) respectively; above-ground dry weight was increased by 19.69% and 17.71%(P<0.05) respectively. By soil drenching with WPS and RA6, plant height and above-ground dry weight were not significantly different from those of control when inoculation rate was low (104 cfu ·g‐1 soil). However when inoculation rate was increased to 108 cfu ·g‐1 soil, seed germination rate was increased by 9.52% and 14.29% respectively(P<0.05); plant height at 15 days after germination was increased by 6.37% and 7.64%(P<0.05); above-ground dry weight was increased by 27.37% and 20.43% (P<0.05). These data suggested that the PGPR strains were potential bio-agents promoting yardlong bean growth. Denaturing gradient gel electrophoresis( DGGE) analysis based on 16S rRNA sequences indicated that microbial communities in PGPR treated soils were obviously different from those in control soil. Strain RA6 specific band became intensified as the time passed. However, strain WP8 specific band became weak as time passed.关键词
植物根际促生菌/豇豆/土著细菌群落Key words
plant growth promoting rhizobacteria( PGPR)/ yardlong bean/ indigenous soil bacterial community分类
资源环境引用本文复制引用
康贻军,沈敏,王欢莉,赵庆新..两株PGPR对豇豆苗期生长及土著细菌群落的影响[J].农业环境科学学报,2012,31(8):1537-1543,7.基金项目
国家自然科学基金(41101235) （41101235）
江苏省高校自然科学基金(10KJB210005) （10KJB210005）
