青岛大学医学院学报2012，Vol.48Issue(3)：250-252,3.
自然流产组织染色体异常的FISH技术检测
DETECTION OF CHROMOSOME ABERRATIONS IN SPONTANEOUS ABORTION BY FISH ANALYSIS
摘要
Abstract
Objective To assess the value of conventional karyotype analysis and FISH in detecting chromosome aberrations in spontaneous abortion materials. Methods G-banding karyotype analysis was carried out after cell culture of spontaneous abortion tissue. The intcrphasc nuclei of 107 abortion tissue were detected by FISH using original DMA probes for chromosomes 13, 16, 18, 21, 22, X and Y, the results of FISH were compared between the two methods. Results Only 76 samples in 107 cases were successfully cultured and G-banding karyotype analysis was conducted, the detection rate being 71. 03%. For FISH technique, all the 107 cases could be done, with 100. 00% detection rate. The difference between the two methods was significant (X2 =36. 25,P<0. 05). In 107 cases of spontaneous abortion, the abnormal samples accounted for 46. 73%, 35 cases were abnormal detected by G-banding, and 48 by FISH, the concordance rate of detection result of the two methods being 96, 05%, Conclusion The detection results of FISH are consistent with the findings of conventional chromosome analysis, but FISH has characteristics of fast and presice, and can be used to analyse massive interphase nuclei, which is in favour of chimeric diagnosis.关键词
原位杂交,荧光/染色体畸变/流产,自然/核型分析Key words
in situ hybridization, fluorescence/ chromosome aberrations/ abortion, spontaneous/ karyotyping分类
基础医学引用本文复制引用
路兴军,俞冬熠,李朔,王修海,王培林..自然流产组织染色体异常的FISH技术检测[J].青岛大学医学院学报,2012,48(3):250-252,3.基金项目
中华医学会分子生物学临床应用研究专项资金(CAMB012010) （CAMB012010）
