青岛大学医学院学报2012，Vol.48Issue(4)：287-290,4.
CD59与CD3在T细胞活化信号转导中的协同作用
THE SYNERGISTIC EFFECT OF CD59 AND CD3 IN T CELL ACTIVATION SIGNALS TRANSDUCTION
摘要
Abstract
Objective To study the synergistic effect of CD59 and CD3 in T cell activation signal transduction, and further explore the mechanism concerning CD59 transmitting signals to T cell. Methods The eukaryotic expression vector containing CD3ζRNAi and CD59 RNAi was identified by enzyme digestion and transfected into Jurkat cells by liposome, while steady cells cloned could get through G418 screening. CD5S and CD3ζ mRNA level were detected by RT PCR. The cell proliferation activity was measured by MTT colorimetry after anti-CD3 mAb and anti-CD59 mAb. The phosphorylation levels of ZAP-70 protein tyro-sine were investigated by immunoblot analysis, the kinetic changes of [Ca2+ ] in T cell were determined by laser scanning confocal microscope imaging, while interleukin 2 (IL-2) in cell supernatants fluid was detected by ELISA. Results CD59 and CD3ζ expression was successfully inhibited in transfected with siRNA plasmid group cells. The cell proliferation, phosphorylation levels of ZAP-70, degree of [Ca2 + ] and IL-2 in transfected siRNA plasmid group cells were decreased compared with non-transfected and Jurkat cells after anti-CD3 mAb and anti-CD59 mAb, the differences were statistically significant (F=96.13-328. 60,q=6. 27 - 32. 664. P<0. 01). Conclusion There is a synergistic effect of CD59 and CD3 in T cell activation signal transduction.关键词
抗原,CD59/抗原,CD3/真核表达载体/Jurkat细胞/信号转导Key words
antigen CD59/ antigen CD3/ eukaryotic expression vector/ Jurkat cells signal transduction分类
基础医学引用本文复制引用
王娟,高美华,张蓓..CD59与CD3在T细胞活化信号转导中的协同作用[J].青岛大学医学院学报,2012,48(4):287-290,4.基金项目
国家自然科学基金资助项目(30972677) （30972677）
