青岛大学医学院学报2012，Vol.48Issue(4)：301-304,4.
尼莫地平对阿糖胞苷诱导HL-60细胞凋亡Caspase信号途径的影响
EFFECT OF NIMODIPINE ON Caspase SIGNAL PATHWAY OF HL-60 CELL APOPTOSIS INDUCED BY CYTARABINE
摘要
Abstract
Objective To study the effect of nimodipine on the mechanism of HL-60 cell apoptosis induced by cytarabine (Ara-c). Methods The log phase HL-60 cells were collected, and divided into control group (no any drugs were aided), nimodipine group (0. 001-0. 100 g/L). Ara-c group (0. 005 - 0. 500 g/L) and nimodipine + Ara-c group. The cells in each group were treated for 24 h. ATP chemiluminometry was employed to detect the cell cytostasis and Caspase 8 activity in each group, and AO/ EB fluorescent staining to observe the cell apoptosis. Results Compared with the control group, the differences of cell proliferation inhibition (PI) rate of HL-60 in different-concentration nimodepine or Ara-c groups were significant (F=12 231. 563, 6 404. 210 iP<0.01). When the concentration of nimodepine was 0. 050 g/L and that of Ara-c was 0. 010 g/L. the PI rate being the highest (F=916. 836,P<0. 01). When the concentration of Ara-c was 0. 010 g/L, and that of nimodepine was 0. 050 g/L, the cell apoptosis rate in both Ara-c and NMDP-t- Ara-c groups was dramatically higher that that in the control group and nimodepine group, the apoptosis being the highest in NMDP+ Ara-c group (F=148. 02,P<0. 01). Compared with the control and nimodipine, the activity of Caspase 8 in Ara-c group and NMDP+ Ara-c group was enhanced, of which, the highest was seen in NMDP+ Ara-c group (F=732. 84,P<0. 01). Conclusion Nimodipine can strengthen HL-60 cell apoptosis induced by Ara-c, the mechanism is probably associated with enhancement of Caspase 8 activity.关键词
尼莫地平/阿糖胞苷/细胞凋亡/HL-60细胞/Caspase 8Key words
nimodipines cytarabine! apoptosiss HL-60 cells/ Caspase 8分类
临床医学引用本文复制引用
王艳华,孙立荣,王玲珍,李晓玲,赵艳霞..尼莫地平对阿糖胞苷诱导HL-60细胞凋亡Caspase信号途径的影响[J].青岛大学医学院学报,2012,48(4):301-304,4.基金项目
山东省自然科学基金资助项目(ZR2010HM130) （ZR2010HM130）
