热带作物学报2012，Vol.33Issue(4)：631-634,4.DOI:10.3969/j.issn.1000-2561.2012.04.009
高效液相色谱法分析巴西橡胶树胶乳再生过程中的ATP、ADP和AMP代谢变化
Analysis of ATP, ADP and AMP Metabolic Change in Laticifers during Latex Regeneration of Hevea brasiliensis by HPLC
摘要
Abstract
The adenosine phosphates level (content of ATP, ADP and AMP) can reflect the real physiological metabolic intensity in laticiferous cells during the latex regeneration. In this paper, a simple and fast high performance liquid chromatographic (HPLC) method was developed for the determination of ATP, ADP and AMP in latex of Hevea brasiliensis, to observe the change of adenosine phosphates during latex regeneration. The results showed that laticiferous cells would synthesize ATP using the residual ADP and AMP at the early stage (0~12 h after tapping). Then the synthesis of AMP, ADP (6 h after tapping) and ATP (12 h after tapping) successively recovered during the latex regeneration and there was an obvious stage of rapid ATP synthesis in the time segment of 12~48 h after tapping. The analysis of energe charge (EC) to the drainage area indicated that, the area of 2~7 cm below the tapping cut in the bark was the sunken region of energe, which was the main place of energe synthesis.关键词
巴西橡胶树/胶乳再生/高效液相色谱/三磷酸腺苷Key words
Hevea brasiliensis/Latex regeneration/HPLC/Adenosine triphosphate分类
农业科技引用本文复制引用
仇键,魏芳,杨文凤,刘实忠,罗世巧,校现周..高效液相色谱法分析巴西橡胶树胶乳再生过程中的ATP、ADP和AMP代谢变化[J].热带作物学报,2012,33(4):631-634,4.基金项目
国家天然橡胶产业技术体系项目(No.CARS-34-GW7) （No.CARS-34-GW7）
中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022011021). （No.1630022011021）
