热带作物学报2012，Vol.33Issue(4)：730-736,7.DOI:10.3969/j.issn.1000-2561.2012.04.028
朱砂根愈伤组织中岩白菜素提取工艺优化
Optimize the Extraction Process of Bergenin in Callus Culture of Ardisia crenata
摘要
Abstract
The effects of different temperature, time and the ratio of solid to solution on the extraction yield of bergenin in Ardisia crenata were investigated based on composite design of quadratic regression. The extraction technique parameters were optimized with response surface methodology, and the results showed that the optimum extraction parameters were the temperature of 80.08 X,, the time of 49.74 minutes and the ratio of solid to solution of 1:10.00. Under those conditions, the concentration of bergenin reached the maximum with 81.34 μg/mL.关键词
朱砂根/愈伤组织/岩白菜素/提取工艺Key words
Ardisia crenata/Callus culture/Bergenin/Extraction process分类
农业科技引用本文复制引用
何雪娇,赖钟雄..朱砂根愈伤组织中岩白菜素提取工艺优化[J].热带作物学报,2012,33(4):730-736,7.基金项目
国家科技支撑计划项目(No.2007BAD07B03) （No.2007BAD07B03）
福建省重大科技平台建设项目(No.2008N2001). （No.2008N2001）
