水产学报2012，Vol.36Issue(7)：993-999,7.DOI:10.3724/SP.J.1231.2012.27815
红色荧光蛋白基因在转基因唐鱼中遗传和表达的稳定性分析
Inheritance and expression stability of red fluorescent protein gene in transgenic (Tanichthys albonubes)
摘要
Abstract
The stability of the red fluorescent protein (RFP) transgenes is important for application of the transgenic Tanichthys albonubes line.This paper investigated comparatively the inheritance and expression of RFP gene in different transgenic T. albonubes generations. Under the observation of fluorescence microscope, RFP gene was expressed in almost all body tissues, and both F6 and F10 generations had a similar expression pattern. Results of self-cross and test-cross breeding experiments of transgenic F6 and F10 individuals indicated that segregation of RFP gene still followed Mendelian single-gene inheritance, and their transgenic progenies had a uniform phenotype. The regions including myosin light polypeptide 2 promoter, RFP gene coding sequence and transgene flanking junctions were amplified by PCR from genomic DNA of F2, F6 and F10 generations. Sequence alignment analysis showed that these important regions were well preserved in transgenic fish, and any kind of alteration at nucleotide level was not found. These results suggested that, RFP gene in transgenic T. albonubes maintained stable inheritance and expression through successive generations.关键词
唐鱼/红色荧光蛋白/基因/表达Key words
Tanichthys albonubes/ red fluorescent protein/ gene/ expression分类
生物科学引用本文复制引用
姜鹏,白俊杰,简清..红色荧光蛋白基因在转基因唐鱼中遗传和表达的稳定性分析[J].水产学报,2012,36(7):993-999,7.基金项目
国家“八六三”高技术研究发展计划(2009AA10Z105) （2009AA10Z105）
广东省科技计划项目(2010B020308002) （2010B020308002）
广东省海洋渔业科技推广专项子项目 (A200899F01) （A200899F01）
