水产学报2012，Vol.36Issue(8)：1159-1166,8.DOI:10.3724/SP.J.1231.2012.27885
牙鲆和半滑舌鳎5S rDNA基因的染色体定位及鲽形目5种鱼类的分子系统学分析
Chromosome mapping of 5S rDNA in olive flounder (Paralichthys olivaceus) and half-smooth tongue-sole (Cynoglossus semilaevis) and molecular systematics analysis of five flatfishes
摘要
Abstract
Using fluorescence in situ hybridization (FISH), 5S rDNA gene was mapped on chromosomes of half-smooth tongue-sole(Cynoglossus semilaevis)and olive flounder(Paralichthys olivaceus)for the first time. The results showed that 5S rDNA has two hybridization signal loci in a pair of homologous chromosomes of male and female half-smooth tongue-sole, respectively; 5S rDNA has two and three hybridization signal loci in a pair of homologous chromosomes of diploid and triploid olive flounder, respectively, referring to the clear and specific hybridization signals. FISH mapping of 5S rDNA genes on the chromosomes of diploid half-smooth tongue-sole and triploid olive flounder provides a feasible method for ploidy and chromosome identification of half-smooth tongue-sole and olive flounder. In addition, the coding region sequences of 5S rDNA gene of turbot (Scophthalmus maximus) and half-smooth tongue-sole were amplified using same primers designed for olive flounder 5 S rDNA gene, and the phylogenetic analysis of the 5S rDNA genes in four species of flatfishes, including plaice(Pleumnectes platessa), senegalese sole (Solea senegalensis), largemouth bass(Micropterus salmoides) and arctic lam-prey (Lampetrajaponica) showed that the 5S rDNA gene of five species of flatfishes shared high homology (98.3%) and gathered into a branch. The GC content was significantly higher than the AT content in conservative sequence of 5S rDNA coding region of these five species of flatfishes.关键词
牙鲆/半滑舌鳎/5S rDNA基因/荧光原位杂交/鲽形目/系统发生Key words
Paralichthys olivaceus/ Cynoglossus semilaevis/ 5S rDNA / FISH/ pleuronectiformes/ phylogeny分类
生物科学引用本文复制引用
谢明树,孙冰,张博,邵长伟,王启龙,王磊,陈松林..牙鲆和半滑舌鳎5S rDNA基因的染色体定位及鲽形目5种鱼类的分子系统学分析[J].水产学报,2012,36(8):1159-1166,8.基金项目
公益性行业(农业)科研专项(200903046) （农业）
山东省泰山学者工程专项 （）
