水产学报2012，Vol.36Issue(8)：1185-1192,8.DOI:10.3724/SP.J.1231.2012.27924
脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析
The genetic diversity and phylogenetic analysis of ITS1 in mitochondrial DNA of three populations of Exopalaemon carinicauda
摘要
Abstract
The ribosomal DNA internal transcribed spacer 1 (ITS 1) of three wild populations of Exopalaemon carinicauda was cloned and sequenced. The phylogenetic relationship among Palaemoninae was discussed using ITS1 from GenBank database. The results show that the length of the ITS1 ranged from 345 to 384 bp. The mean contents of GC were higher than AT;79 variation sites and 39 haplotypes were obtained in ITS 1; Nucleotide diversity and average number of nucleotide diferences were calculated to analyze the gene sequence variation. Eight kinds of microsatellites were found in E. carinicauda. The type of (GA)n was rich in ITS1. AMOVA analysis results show that the genetic differentiation of the three groups was weak or only moderately differentiated.The molecular phylogenetic tree was constructed with NJ method using software MEGA 4.0 to study several species of the Palaemoninae molecular phylogeny. According to the NJ tree, different individuals of the same species, different species of the same genera were grouped together, in consistence with the morphological taxonomy.关键词
脊尾白虾/ITS1/遗传多样性/系统进化Key words
Exopalaemon carinicauda/ ITS1/ genetics diversity/ phylogenetic分类
生物科学引用本文复制引用
马朋,刘萍,李健..脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析[J].水产学报,2012,36(8):1185-1192,8.基金项目
国家“八六三“高技术研究发展计划(2012AA10A409) （2012AA10A409）
国家虾产业技术体系(CARS-47) （CARS-47）
山东省科技发展计划项目(2011GHY11526) （2011GHY11526）
