水产学报2012，Vol.36Issue(8)：1304-1312,9.DOI:10.3724/SP.J.1231.2012.27915
水杨酸对龙须菜抗高温生理的影响
Effects of salicylic acid on the resistance of Gracilaria/Gracilariopsis lemaneiformis to high temperature
摘要
Abstract
The marine red algae Gracilaria/Gracilariopsis lemaneiformis were used as materials to study the effects of different concentration of salicylic acid on their growth and stress physiology under high temperature condition. The antioxidant enzyme activity, the osmotic regulation substances, phycobilipro-tein, the features of chlorophyll fluorescence and HSP70 gene were studied when the algae grew in high temperature environment. Results showed that in the salicylic acid treatment group, the growth rate, enzymatic activity or membrane lipids damage degree were better than those of the control group at various degrees, and 5.0 and 10.0 μg/mL of salicylic acid treatment groups were obviously better than the other groups. The treatment with 10.0 ug/mL of salicylic acid showed the best effect with the daily relative growth rate increased 340% compared with the control group. When the treatment was on the 3rd day, each index of the 10.0 ug/mL group reached the maximum value, SOD increased 74%, POD increased 70%, CAT increased 40%, mannitol and proline increased 70% and 26% respectively. The content of phyco-erythrin increased 46.2%, phycocyanin content increased 40%. Compared with the control group, the decrease of MDA content on the first day was the most obvious and decreased nearly 10%. The chlorophyll parameters of Fv/Fm, Fv/Fo, qP and ΦBPS Ⅱ of G. lemaneiformis decreased under high temperature stress, and the variation tendency of NPQ increased first and then declined. It was found that 10.0 ug/mL of salicylic acid could increase the five kinds of chlorophyll fluorescence values compared with the control group. The expression level of HSP70 gene under high temperature was higher than those under normal conditions, while the expression level of high temperature group with salicylic acid treatment was lower than the control group. In conclusion, salicylic acid showed the effect of improving the high temperature resistance of G. lemaneiformis.关键词
龙须菜/水杨酸/逆境生理/叶绿素荧光/HSP70基因Key words
Gracilaria/Gracilariopsis lemaneiformis/ salicylic acid/ stress physiology/ chlorophyll fluorescence/ HSP70 gene分类
农业科技引用本文复制引用
朱招波,孙雪,徐年军,骆其君..水杨酸对龙须菜抗高温生理的影响[J].水产学报,2012,36(8):1304-1312,9.基金项目
国家自然科学基金项目(31072229) （31072229）
浙江省钱江人才项目(2007R10038) （2007R10038）
