四川中医2012，Vol.30Issue(7)：55-57,3.
HPLC法同时测定陈皮中4个黄酮类成分的含量
The Contents of Four Flavonoid Components in Tangerine Peel were Simultaneously Determined by HPLC
肖建春 1陈帅 1袁崇均 1罗森 1吴燕 1王笳1
作者信息
- 1. 四川省中医药科学院 四川 成都 610041
- 折叠
摘要
Abstract
Objective; To establish an HPLC method for simultaneous determination of hesperidin, naringin, nobiletin and tangeretin in tangerine peel. Methods; The RP-HPLC system consisting of Cl8 (150mm×4.6mm, 5μm) column and a solution system of acetonitrile-water, gradient elution: 0→30min, the proportion of 5; 95→25:75; 30→55min, the ratio of 25:75 →95: 5; 95: 5 ratio of run to 60 min. The column temperature was 30℃, detection wavelength; 283nm, flow rate 0. 8ml/ min. Results; The linear ranges of hesperidin, naringin, nobiletin and tangeretin respectively were 0.4632 ~4. 632μg (r = 0.9999), 0.5869-5. 869μg (r=0.9998), 0.4439 ~ 4. 439 μg (r = 0.9998), and 0.4065-4.065 μg (r = 0.9997). The average recoveries ( n = 6 ) respectively were 98. 67% ( RSD = 1.97% ), 98. 33% ( RSD = 1. 85% ) , 98. 05% ( RSD = 1.76%), and 98.46% (RSD =2.05%). Conclusions; The method is quick, simple and accurate with good reproducibility, and can be used for determination of hesperidin, naringin, nobiletin and tangeretin in tangerine peel and quality control of tangerine peel.关键词
反相高效液相色谱法/陈皮/黄酮Key words
RP-HPLC/ Tangerine peel/ Flavonoids分类
医药卫生引用本文复制引用
肖建春,陈帅,袁崇均,罗森,吴燕,王笳..HPLC法同时测定陈皮中4个黄酮类成分的含量[J].四川中医,2012,30(7):55-57,3.
