HPLC法测定清开灵注射液中栀子苷的含量OACSTPCD
Determination of geniposide in gardenia of Qingkailing injection by HPLC
目的:建立测定清开灵注射液中栀子苷含量的高效液相色谱方法,为栀子药材及其制剂的质量控制提供依据.方法:采用高效液相色谱法,色谱柱为默克C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-水(15:85),检测波长238 nm,流速为1.0 mL/min,柱温30℃.结果:栀子苷在4～ 280 ng与峰面积呈良好的线性关系(r=0.9995),平均回收率100.37％,平均RSD 0.73％.结论:高效液相色谱法测…查看全部>>
Objective:Establishing a HPLC method for the content determination of geniposide in gardenia and providing valuable data for quality control of gardenia and its preparations such as Qingkailing injection. Methods:The sample were separated on a Merck lichrosphere column(250 mm × 4.6 mm,5 μm) column with mobile phase of ace-tonitrile-water( 15:85) in the flow rate of 1.0 mL/min. The detection wave length was set at 238 nm. Column temperature was 30℃. Results:T…查看全部>>
孙秀玉;王英姿;贺葵邦;张胜海;冯艾灵
北京中医药大学中药学院,北京100102北京中医药大学中药学院,北京100102北京中医药大学中药学院,北京100102北京中医药大学中药学院,北京100102北京中医药大学中药学院,北京100102
医药卫生
栀子栀子苷清开灵注射液高效液相色谱法含量测定
gardeniageniposideQingkailing injectionHPLCcontent determination
《山东中医药大学学报》 2012 (4)
352-353,2
国家重大新药创制基金资助(编号:2010ZX09502-002)北京中医药大学复方中药制药研究创新团队项目资助(编号:2011-CXTD-13)
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