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不同花生品种胚小叶芽诱导的研究

任艳 李磊 崔潇 石延茂 王辉 李双铃 袁美

山东农业科学2012,Vol.44Issue(7):13-15,3.
山东农业科学2012,Vol.44Issue(7):13-15,3.

不同花生品种胚小叶芽诱导的研究

Study on Shoot Induction from Leaflets of Different Peanut Cultivars (Arachis hypogaea L.)

任艳 1李磊 2崔潇 3石延茂 3王辉 2李双铃 2袁美2

作者信息

  • 1. 中国海洋大学,山东青岛266100
  • 2. 山东省花生研究所,山东青岛266100
  • 3. 青岛市农业科学研究院,山东青岛266100
  • 折叠

摘要

Abstract

The shoot induction rate of lobules embryos from 4 peanut cultivars were compared in the media added different concentrations of NAA and TDZ. The sterilizing effect of 0.1% HgC12 and 10% NaC1O and their influences to seed germination were also observed. The results indicated that the sterilizing effect was ideal and the germination rate was higher when the peanut seeds were treated for 0. 5 - 1.0 minutes with alcohol and 15 minutes with 10% NaCIO. The media added 1 mg/L NAA and 0.02 mg/LTDZ were the most favorable to in vitro shoot induction with the induction rate over 80%. In which, the shoot induction rate of Hu-ayu 20 was the highest as 89.2%.

关键词

花生品种/组织培养/种子萌发/芽诱导

Key words

Peanut cultivar/ Tissue culture/ Seed germination/ Shoot induction

分类

农业科技

引用本文复制引用

任艳,李磊,崔潇,石延茂,王辉,李双铃,袁美..不同花生品种胚小叶芽诱导的研究[J].山东农业科学,2012,44(7):13-15,3.

基金项目

科技部"863"计划项目(2006AA100106) (2006AA100106)

山东农业科学

OACSTPCD

1001-4942

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