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硝酸镧、L-苯丙氨酸对半夏愈伤组织生长和总生物碱积累的影响

曹孝鲍 王震 彭爽 王小敏 张瑞 叶宝兴

山东农业科学2012,Vol.44Issue(7):26-28,3.
山东农业科学2012,Vol.44Issue(7):26-28,3.

硝酸镧、L-苯丙氨酸对半夏愈伤组织生长和总生物碱积累的影响

Effects of Lanthanum Nitrate and L-phenylalanine on Callus Growth and Total Alkaloid Accumulation of Pinellia ternata

曹孝鲍 1王震 1彭爽 1王小敏 1张瑞 1叶宝兴1

作者信息

  • 1. 山东农业大学作物生物学国家重点实验室/山东农业大学生命科学学院,山东泰安271018
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摘要

Abstract

The effects of lanthanum nitrate as elicitor and L - phenylalanine as precursor on callus growth rate and alkaloid content of Pijiellia ternata were studied in this paper. AfteT cultured for 38 days, the results showed that they had no significant effects on the callus growth rate and total alkaloid content of Pinellia ternata. The content of alkaloid was the most when the concentration of lanthanum nitrate and L - phenylalanine were 2.25 mg/L and 6.00 mg/L respectively, which increased by 76. 89% and 8.99% respectively compared with the check. With the increase of the added lanthanum nitrate and L - phenylalanine, the callus growth rate and alkaloid accumulation showed the tread of increasing firstly and then decreasing.

关键词

半夏/硝酸镧/L-苯丙氨酸/愈伤组织/生物碱

Key words

Pinellia ternata/ Lanthanum nitrate/ L - phenylalanine/ Tissue culture/ Alkaloid

分类

农业科技

引用本文复制引用

曹孝鲍,王震,彭爽,王小敏,张瑞,叶宝兴..硝酸镧、L-苯丙氨酸对半夏愈伤组织生长和总生物碱积累的影响[J].山东农业科学,2012,44(7):26-28,3.

基金项目

山东农业大学大学生科研训练计划(SRT)资助(1112013). (SRT)

山东农业科学

OACSTPCD

1001-4942

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