山东医药2012，Vol.52Issue(24)：4-6,后插1,4.
补脾益气方药对哮喘大鼠嗜酸性粒细胞趋化因子表达的影响
Effect of traditional Chinese medicine prescription for Reinfocing Pi Qi on the expression of eotaxin in asthmatic rat model
摘要
Abstract
Objective To observe the intervention influence of traditional Chinese medicine (TCM) prescription for Reinfocing Pi Qi on the expression of eotaxin in asthmatic rat, and to explore its possible mechanism. Methods SPF male SD rats were randomly divided into normal control group (A group) . Asthmatic group (B group), normal saline group (C group) , therapeutic group of Dexamethasone (D group), therapaeutic group of TCM prescription for Reinforcing Pi Oi (E group). The asthmatic models were established by the classic way of OVA sensilization and excitation. Dexamethasone and normal saline were used as positive and negative control medicine. TCM prescription for Reinforcing Pi Qi were taken once a day in 1 mL/100 g for 14 days after seting up model in the rats of E group. The number of total cells, percentages of eo-sinophil (EOS), lymphocyte, neutrophil in bronchoaleolar lavage ( BALF) fluid were calculated. Pathological change of lung tissue were observed. The expression of eotaxin on level of mRNA and protein in lung tissue were detected by RT-PCR and Western blot assay. Results EOS number of BALF and expression of eotaxin in B group and C group were higher than those in A group (P < 0.01). EOS number of BALF and expression of eotaxin in D group and E group were lower than those in B group (P < 0.01). However, there was no difference between D group and E group ( P > 0.05). Conclusion TCM prescription for Reinforcing Pi Qi can decrease the level of eotaxin in asthmatic rats, and alleviate inflammatory cells infiltration in lung, then inhibit airway inflamation.关键词
补脾益气方药/嗜酸性粒细胞趋化因子/哮喘/大鼠Key words
traditional Chinese medicin prescription for Reinfocing Pi Qi/ eotaxin/ asthma/ rat分类
医药卫生引用本文复制引用
许珂玉,王艳杰,赵丹玉,柳春..补脾益气方药对哮喘大鼠嗜酸性粒细胞趋化因子表达的影响[J].山东医药,2012,52(24):4-6,后插1,4.基金项目
辽宁省教育厅高等学校科研项目计划(2009A500). （2009A500）
