山东医药2012，Vol.52Issue(24)：7-9,3.
辛伐他汀对CRP诱导变异型心绞痛患者NF-κB合成的影响
Effect of simvastatin on nuclear factor-κB synthesis in patients with variant angina stimulated by C reactive protein
摘要
Abstract
Objective To investigate whether nuclear factor-κB ( NF-kB) might be involved in the pathogenesis of variant angina (VA) and the effect of simvastatin on C-reactive protein (CRP) -dependent inflammatory activation in mono-cytes of patients with V A. Methods Monocytes of patients with V A (VA, n = 6 ) and normal controls ( controls, n = 6) were cultured and stimulated with 20 mg/L of CRP for 24 hours. Also 10 μmol/L of simvastatin was pre-incubated for 2 hours with monocytes in the presence of CRP. The levels of NF-kB were evaluated by enzyme-linked immunosorbent assay (ELISA). Results The levels of NF-kB in baseline status were similar in patients with VA and normal controls. The levels of NF-kB induced by CRP were significantly elevated both in VA and normal controls compared with their baseline status ( P <0.01). Patients with VA were detected to have significantly higher NF-kB levels induced by CRP in comparison with normal controls (P < 0.01). Simvastatin significantly inhibited the production of NF-kB in monocytes stimulated by CRP both in VA and in normal controls(P<0.01). Conclusions CRP could induce more NF-kB release in human monocytes of patients with VA than normal controls, suggesting that an enhanced inflammatory response to CRP may be involved in the pathogenesis of VA. Simvastatin could inhibit this response which could provide an insight into the machnisins of anti-inflammation in statins.关键词
冠状动脉疾病/变异型心绞痛/C反应蛋白/单核细胞/NF-κBKey words
coronary disease/ variant angina/ C-reactive protein/ monocyte/ NF-kappa B分类
医药卫生引用本文复制引用
朱成刚,李建军,刘俊,郭远林,吴娜琼,李小林,唐熠达,蒋立新..辛伐他汀对CRP诱导变异型心绞痛患者NF-κB合成的影响[J].山东医药,2012,52(24):7-9,3.基金项目
中国医学科学院院所青年基金(2010-F23) （2010-F23）
教育部博士点专项基金(20111106110013). （20111106110013）
