山东医药2012，Vol.52Issue(25)：9-11,3.
人参皂苷Rg1联合BMSCs对大鼠脑缺血再灌注损伤的保护作用
Effects of ginsenoside Rg1 joint BMSCs on cerebral ischemia and reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effect of ginsenoside Rgl jointed BMSCs on ischemia and reperfusion injury in rats. Methods A total of 25 SD rats were divided into normal control group (group A) , model group ( group B) , BMSCs treatment group ( group C) , ginsenoside Rgl treatment group ( group D) , BMSCs and ginsenoside Rgl combination therapy group ( group E) , each group 5 rats. Model of cerebral ischemia and reperfusion was established by the method of middle cerebral artery occlusion. Intraventricular transplantation was done after BrdU labeling BMSCs. After 4W, single-blinded neurological deficit scores was detected. The expressions of BrdU and NSE protein were detected by Nissl staining counted cortical neurons and immunohistochemical staining. Results There was no neurological deficit symptoms in Group A . Deep purple Nissl bodies can be seen in the cortical neurons of the cytoplasm. It showed a significant neurological deficit symptoms in group B. Nissl bodies were significantly reduced or even disappear. Compared with group B, the neuronal number in the group C, D, E increased, especially in group E. BrdU positive cells can be seen a-round the infarcted area in group C and E. Expressions of NSE in group C, D and E were higher than those in group B, especially in group E. Conclusion Ginsenoside Rgl jointed BMSCs can promote the repair of cerebral ischemia and reperfusion injury.关键词
人参皂苷Rg1/骨髓间充质细胞/脑缺血再灌注/大鼠Key words
ginsenoside Rgl/ bone marrow mesenchymal cells/ cerebral ischemia and reperfusion/ rats分类
医药卫生引用本文复制引用
包翠芬,包翠芳,李磊,刘霞..人参皂苷Rg1联合BMSCs对大鼠脑缺血再灌注损伤的保护作用[J].山东医药,2012,52(25):9-11,3.基金项目
辽宁省教育厅创新团队项目(LT2010061) （LT2010061）
辽宁省自然基金项目(201102139) （201102139）
辽宁医学院博士启动基金项目(Y20101303). （Y20101303）
