山东医药2012，Vol.52Issue(26)：3-6,4.
人胶质瘤细胞及组织中miRNA-221、miRNA-222表达变化及意义
Expression change and significance of miRNA-221/222 in glioma cell and tissue
摘要
Abstract
Objective To observe the expression changes of miRNA-221, miRNA-222 in human glioma cell and tissue , of which correlation with the radiosensitivity of human glioma was discussed. Methods The total RN A was extracted from in-vitro glioma cell lines U87, U251, A172, LN229 and 60 cases of glioma tissue (collected through operations) according to Trizol kit. The expression of miRNA-221 and miRNA-222 were detected with real-time PCR, and normal brain tissue was control sample . 60 cases of glioma patients was treated with radiotherapy after surgery. Glioma patients were fol-lown-up and the radiotherapy in gliomas were analized by MRI examination. Glioma cell survival rate after X-ray irradiation (irradiation dose of 2 Cy) was estimated by cloning experiments, with gastric mucosal epithelium cells as control. Results Compared with the control group, miRNA-221, miRNA-222 in glioma cell lines and tissue were highly expressed (all P < 0.05 ) . The expression of miRNA-221, miRNA-222 in high grade glioma was significantly higher than the low grade( all P<0.05).The SF of glioma cell was significantly higher than that of ges after irradiation (allP<0.05). MiRNA-221 and miRNA-222 expression in glioma cell was positively correlated with glioma SF after X-ray radiation( r = 0.673,0.696, all P <0.05). Glioma patients with miRNA-221, miRNA-222 highly expressed had a short survival time than the patients with miRNA-221, miRNA-222 low expression (all P < 0.05). Conclusions MiRNA-221 and miRNA-222 highly express in glioma cell and tissue, their expression was positively correlated with the radiation resistance of glioma cell, and their expression in glioma tissue are negatively correlated with the patients' survival time.关键词
颅内肿瘤/胶质瘤/微小RNA/放射敏感性/放射抗性Key words
intracranial tumors/ glioma/ microRNA/ radiation resistance分类
医药卫生引用本文复制引用
魏厚禄,张春智,戚贵军,姚鑫,陈步东,孙树鹏,王计伟,梁思泉,杨玉山..人胶质瘤细胞及组织中miRNA-221、miRNA-222表达变化及意义[J].山东医药,2012,52(26):3-6,4.基金项目
国家自然科学基金资助项目(30901772). （30901772）
