山东医药2012，Vol.52Issue(26)：20-22,3.
替莫唑胺对大鼠垂体腺瘤GH3细胞增殖及其激素分泌的影响
Effect of temozolomide on GH3 pituirary ademomas cell proliferation and hormonal production
摘要
Abstract
objective To evaluate the effect of temozolomide(TMZ) on GH3 pituirary ademomas cell proliferation, apoptisis and hormonal production. Methods The different dose of TMZ was added to GH3 cells cultured in vitro, the control group was added with DMSO diluent, after administration of 24, 48, 72, 96 h, cell proliferation was assessed by MTT assay, apoptosis was detected, by TUN'EL staining, the change of prolactin secretion was measured by ELISA. Results TMZ could inhibit CH3 cell proliferation which depended on the time and concentration of treatment significantly (P < 0.05). GH3 cell apoptosis with added TMZ was significantly higher compared to control group (P < 0.05). After 24 h administration of TMZ, the prolactin secretion of GH3 cell was lower compared to control group. Conclusions TMZ can inhibit GH3 pituirary ademomas cell proliferation and induce apoptosis and supress hormonal secretion, these benefits prove that TMZ could be used in the management of aggressive pituitary ademoma treatment.关键词
替莫唑胺/垂体腺瘤/细胞凋亡/泌乳素Key words
temozolomide/pituitary ademoma/apoptosis/prolactin分类
医药卫生引用本文复制引用
白敬洋,衣服新,马俊,常锐..替莫唑胺对大鼠垂体腺瘤GH3细胞增殖及其激素分泌的影响[J].山东医药,2012,52(26):20-22,3.基金项目
辽宁省教育厅科研基金资助项目(L2010317) （L2010317）
辽宁省科学技术计划项目(2010225034). （2010225034）
