山东医药2012，Vol.52Issue(28)：3-6,4.
不同转移潜能大肠癌细胞株基因表达谱的差异分析
Analysis gene expression profiling in different metastatic potential colon cancer cell lines
摘要
Abstract
Objective To analysis characteristics of different metastatic potentials colorectal cancer cell lines through gene expression profiling and explore the pro-organ metastasis of colorectal cancer molecular mechanisms. Methods Affy-tnetrix HG-U133 plus 2.0 situ synthesized oligonucleotide microarrays were the same parent with different metastatic potential and organ affinity of metastasis in colorectal cancer cell lines SW620, SW480 and SW480 liver metastasis gene expression profiles, SW4S0 as the control cell lines, screening of " colorectal cancer metastasis-related genes" and " pro-organ metastasis-related genes" and the application of GOTM software for analysis of gene function. Results Screening out two groups of SW620, SW480 liver metastasis co-enpression of colorectal cancer metastasis-related genes were 422, " pro-organ metastasis-related genes or ESTs" were 3 054. " Metastasis-related genes & ESTs" functions mainly related to cell metabolism, cellular physiological process, regulation, signal transduction, macromolecule metabolism, metabolism and basic metabolism and so on. The CO analysis of "pro-organ metastasis-related genes or ESTs" showed that SW620, SW480 liver metastasis functional classification of cell line showed differences in cell adhesion, signal transduction, cell motility, regulate enzyme activity, nucleic acid metabolism and metal ion binding and so on. Conclusion Colorectal cancer cell lines with different metastatic potential through gene expression profiles analysis of colorectal cancer metastasis might help us to understand closely the molecular mechanisms of organ affinity of metastasis-related genes and screening.关键词
大肠癌/基因表达谱/转移相关基因Key words
colorectal cancer/ gene expression profiling/ metastasis-related genes分类
医药卫生引用本文复制引用
余力,王瑜,刘莉,丁彦青..不同转移潜能大肠癌细胞株基因表达谱的差异分析[J].山东医药,2012,52(28):3-6,4.基金项目
广东省自然科学基金资助项目(9151503101000016). （9151503101000016）
