山东医药2012，Vol.52Issue(31)：23-25,3.
氯胺酮对乳鼠海马CA3区神经元凋亡及NMDAR-2B表达的影响
Effects of ketamine on the neurons apoptosis and NMDAR-2B expression in neonatal rats hippocampal CA3 area
摘要
Abstract
Objective To investigate the effects of ketamine on the neurons apoptosis and N-methyl-D -aspartate receptor (NMDAR)-2B expression in neonatal rats hippocampal CA3 area. Methods Twelve postnatal 7 days SD rats were randomly divided into control group and ketamine group with 6 rats in each. The ketamine group was treated with intraper-itoneal injection of 20 mg/kg ketamine interval of 90 mins, for a total of five times. The control group was treated with same volume of normal saline at corresponding time points.After 24 hours, the rats were fixed by perfusion; hippocampal neuro-nal apoptosis was detected by TUNEL method; immunohistochemical method was used to detect the expression of NMDAR-2B in CA3 area of hippocampal neurons. Results Compared with the control group, the number density of apoptotic cells and NMDAR-2B expression were both significantly increased (P <0, 05). Conclusion Ketamine can increase apoptosis of neonatal rats hippocampal neurons, which maybe induced by the upregulation expression of NMDA receptor.关键词
氯胺酮/细胞凋亡/N-甲基-D-天冬氨酸受体Key words
ketamine/ apoptosis/ N-methyl-D -aspartate receptor分类
医药卫生引用本文复制引用
石永勇,贾彬,招伟贤,李向宇,古妙宁..氯胺酮对乳鼠海马CA3区神经元凋亡及NMDAR-2B表达的影响[J].山东医药,2012,52(31):23-25,3.基金项目
广东省自然科学基金资助项目(No:07004823) （No:07004823）
广州中医药大学创新基金(No:10CX041) （No:10CX041）
广东省医学科研基金(No:B2011129). （No:B2011129）
