上海农业学报2012，Vol.28Issue(3)：32-36,5.
3种番茄斑萎病毒属病毒多重RT-PCR检测方法的建立
A method of synchronously detecting three kinds of Tospovirus viruses by multiplex RT-PCR
摘要
Abstract
The most conserved regions were selected via aligning the total genomic sequences of tomato spotted wilt virus(TSWV) ,Impatiens necrotic spot virus(INSV)and iris yellow spot virus(IYSV) in the Genebank by using the LaserGene software, the viruses'specific primers were designed,and a method of detecting TSWV,INSV and IYSV by multiplex RT-PCR was established through progressively adjusting and optimizing the PCR conditions. The results showed that the method could synchronously detect TSWV,INSV and IYSV from an about-16 μg infected tobacco leaf and had relatively high specificity and accuracy. The method could detect TSWV,INSV and IYSV of infected host plants synchronously,rapidly and specifically.关键词
病毒检测/番茄斑萎病毒/凤仙花坏死斑病毒/鸢尾黄斑病毒/多重RT-PCRKey words
Virus detection/ Tomato spotted wilt virus/ Impatiens necrotic spot virus/ Iris yellow spot virus (Multiplex RT-PCR分类
农业科技引用本文复制引用
代欢欢,陈舜胜,杨翠云,于翠..3种番茄斑萎病毒属病毒多重RT-PCR检测方法的建立[J].上海农业学报,2012,28(3):32-36,5.基金项目
国家质检总局科技项目(20111K172)资助 （20111K172）
