上海海洋大学学报2012，Vol.21Issue(3)：368-373,6.
条斑紫菜藻红蛋白粗提方法比较
Comparison of crude extraction methods of phycoerythrin from Porphyra yezoensis
摘要
Abstract
We compared the methods of crude phycoerythrin extraction from Porphyra yezoensis, a main economic red alga in China. Porphyra yezoensis thallus was broken with ＂swelling ＆ smash＂ method, and crude extract was dealed in sequence with chemical reagent such as activated carbon, rivanol or physical condition such as isoelectric point, concentration, pH, temperature and differential centrifugation. It showed that the purity of phycoerythrin increased when activated carbon was heavily applied alone. When usage of activated carbon achieved two percent of that of Porphyra, the effect was best. And this method was much better than combined using of activated carbon and rivanol on a small scale. On the other hand, ammonium sulfate precipitation combined with pH regulating increased the purity, which was in line with concentration of solution and arrived at topmost purity of 1.35, which achieved 1.42 after differential centrifugation followed. While isoelectric precipitation or temperature control decreased the purity. This research laid the groundwork for preparation of phycoerythrin from Porphyra yezoensis.关键词
条斑紫菜/藻红蛋白/活性碳/利凡诺/差速离心Key words
Porphyra yezoensis/phycoerythrin/activated carbon/rivanol/differential centrifugation分类
生物科学引用本文复制引用
蔡春尔,李春霞,藤一悦,林子龙,顾碧莹,何培民..条斑紫菜藻红蛋白粗提方法比较[J].上海海洋大学学报,2012,21(3):368-373,6.基金项目
国家高技术研究发展项目 （）
国家科技支撑计划课题 （）
上海市教育委员会重点学科建设项目 （）
