同济大学学报（医学版）2012，Vol.33Issue(3)：1-4,4.DOI:10.3969/j.issn1008-0392.2012.03.001
Bcl-2高表达小鼠模型的建立
Establishment of mouse model highly expressing Bcl-2 protein
摘要
Abstract
Objective To establish a mouse model highly expressing Bcl-2 protein for study of Bcl-2 function in vivo. Methods The plasmid containing human bcl-2 gene was constructed, which was transferred to fertilized eggs of C57BL/6J × CBA hybrid mice by microinjection; the fertilized eggs were implanted in the uterus of falsely pregnant female mice. The bcl-2 gene-carrying mice were the "Founder" mice, the "Founder" mice and C57BL/6J × CBA hybrid mice were continuously backcrossed, and the transgenic mice expressing Bcl-2 were obtained by screening with PCR and Western blotting. Results Five "Founder" mice were obtained; the strains were successfully established from #6 and #14 mice. After 7 generations of backcross breeding and screening by PCR and Western blot, 42 transgenic mice highly expressing bcl-2PCR were obtained. Conclusion The mouse model highly expressing Bcl-2 protein has been successfully established in this study.关键词
Bcl-2蛋白/转基因/高表达/小鼠Key words
Bcl-2 protein/transgene/high expression/mouse分类
医药卫生引用本文复制引用
张燕飞,降建新,王瑛,张夔鸣,范慧敏,孙志扬..Bcl-2高表达小鼠模型的建立[J].同济大学学报（医学版）,2012,33(3):1-4,4.基金项目
上海市浦东新区社发局科研基金(PW08-07) （PW08-07）
