同济大学学报（医学版）2012，Vol.33Issue(3)：20-24,5.DOI:10.3969/j.issn1008-0392.2012.03.005
膳食锌缺乏对阿尔茨海默病模型鼠大脑内锌转运体mRNA表达的影响
Effect of dietary zinc deficiency on mRNA expression of zinc transporters in the brain of transgenic mice of Alzheimer's disease
黄昕 1葛懿云 1张怡琼 1李俊旋 1谢良民1
作者信息
- 1. 同济大学附属东方医院营养科,上海200120
- 折叠
摘要
Abstract
Objective To investigate the effect of dietary zinc deficiency on the mRNA expression of ZnT-1, MT1 and ZIP1 in the brain of mice with Alzheimer's disease. Methods Thirty three adult APPswe/PSEN1dE9 double transgenic mice(Alzheimer's disease model) were randomly divided into dietary zinc deficiency group 1 (ZD1, n = 10) , dietary zinc deficiency group 2( ZD2, n = 10) , dietary zinc supplement group (ZS, n = 8) and the positive control group (PC, n =5) , with 10 normal mice as a negative control( NC). ZnT-1, MT1 and ZIP1 mRNA expression in the brain were detected by RT-PCR. Results ZnT-1 mRNA expression was significantly increased in group PC compared with group NC(P <0.01), MT1 and ZIP1 mRNA expression in the group PC and group NC had no significant differences. Compared to group PC, ZnT-1 expression was significantly decreased in group ZD1 and more markedly decreased in group ZD2 ( P < 0. 01). The expression of ZnT-1 mRNA was increased with the amount of dietary zinc supplemented in a time- and dose-response manner. There were no significant changes of MT1 and ZIP1 mRNA expression in groups ZD1 and PC; but the expression of MT1 and ZIP1 mRNA was significantly decreased in group ZD2 after 80 days of feeding with zinc deficiency diet(P <0.01). Conclusion ZnT-1 mRNA is up-regulated in the brain of adult APPswe/ PSENldE9 double transgenic mice, and dietary zinc supplement can down-regulate the expression of ZnT-1, MT1 and ZIP1 mRNA in the brain.关键词
膳食锌/APPswe/PSEN1dE9双转基因小鼠/锌转运体/阿尔茨海默病Key words
dietary zinc deficiency/ APPswe/PSEN1dE9 double-transgenic mice/ zinc transporter/ Alzheimer's Disease分类
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黄昕,葛懿云,张怡琼,李俊旋,谢良民..膳食锌缺乏对阿尔茨海默病模型鼠大脑内锌转运体mRNA表达的影响[J].同济大学学报（医学版）,2012,33(3):20-24,5.
