同济大学学报（医学版）2012，Vol.33Issue(4)：13-18,6.DOI:10.3969/j.issn1008-0392.2012.04.003
尿多酸肽诱导急性早幼粒细胞白血病细胞株凋亡的实验研究
Uroacitides induce apoptosis of acute promyelocytic leukemia cells in vitro
周宏 1万海英 1李冬1
作者信息
- 1. 同济大学附属同济医院检验科,上海200065
- 折叠
摘要
Abstract
Objective To investigate the apoptosis-inducing effect of Uroacitides(CDA- II) on acute promyelocytic leukemia (APL) cell line NB4 and NB4-R1 (retinoic acid resistant). Methods The cultured NB4 and NB4-R1 cells were treated with different concentrations of CDA- II. The changes of cell morphology were observed; cell proliferation and growth inhibition was measured by cell counting; apoptosis was examined by flow cytometry; the activation of protein kinase C (PKC8) and Caspase-3 was detected by Western blotting. Results After treated by CDA- II the growth of NB4 and NB4-R1 cells was inhibited; morphological differentiation and apoptosis were observed. Annexin V-PI method showed that cell apoptosis rates were increased to (58.7 ± 3.1)% and (87.8 + 0.5)% for NB4 and NB4-R1 cells respectively after 24 h of treatment, and PKC8 and Caspase-3 were activated. Conclusion CDA- E can induce apoptosis of NB4 and NB4-R1 cells by activating the protein Caspase-3 and PKC5.关键词
尿多酸肽/急性早幼粒细胞白血病/细胞凋亡/维甲酸耐药Key words
Uroacitides/ acute promyelocytic leukemia/ apoptosis/ retinoic acid resistant分类
医药卫生引用本文复制引用
周宏,万海英,李冬..尿多酸肽诱导急性早幼粒细胞白血病细胞株凋亡的实验研究[J].同济大学学报（医学版）,2012,33(4):13-18,6.
