生命科学仪器2012，Vol.10Issue(4)：17-20,4.
高效液相色谱-荧光检测方法用于单胺氧化酶抑制剂抑制效果的评价
HPLC-Fluorescence Detection Used for Evaluating the Effect of Monoamine Oxidase Inhibitor
摘要
Abstract
Monoamine oxidase （MAO, EC 1.4.3.4） is an important membrane-bound flavoenzyme that can modulate the metabolism of The research of its inhibitor plays an important role in treating depressive disorder and other neurodegenerative diseases. In this study, we established a new method to determine the efficiency of the inhibitor using HPLC- Fluorescence Detector. In this study, the monoamine oxidase was obtained from liver of Wistar male rat, and Kynuramine was used as substrate. We measured the enzyme repression of Pargyline and Clogyline that the commonly medicine used to treat mental disorder. Compared to other methods, this approach manipulates simple and safety, can detect the inhibition efficiency quickly.关键词
单胺氧化酶/犬脲胺/抑制剂/高效液相色谱/荧光检测Key words
Monoamine oxidase (MA0)/Kynuramine/Inhibitor/HPLC/Fluorescence Detection分类
生物科学引用本文复制引用
王珊珊,马培翔..高效液相色谱-荧光检测方法用于单胺氧化酶抑制剂抑制效果的评价[J].生命科学仪器,2012,10(4):17-20,4.基金项目
研究基金来源：十一五科技支撑项目 （）
