中国生态农业学报2012，Vol.20Issue(8)：1048-1053,6.DOI:10.3724/SP.J.1011.2012.01048
转EdHP1(氢离子焦磷酸化酶)基因小麦的钾利用特征研究
Potassium utilization in EdHP1 (H+-pyrophosphatase) transgenic wheat
摘要
Abstract
With intensive land farming, soil potassium deficiency has become increasingly serious, limiting sustainable agricultural development. In this study, the differences between EdHP1 (H+-pyrophosphatase) transgenic and wild wheat were investigated in terms of potassium absorption and utilization, rhizosphere soil potassium dynamic and soil enzyme activities. The research aimed to determine the potentials of transgenic technologies in promoting wheat potassium absorption and utilization. The results showed that available K in rhizosphere of transgenic wheat was significantly lower than that of wild wheat at different K-levels. Available K in rhizosphere of transgenic wheat were 8.30% (30 d), 15.02% (60 d) and 12.53% (75 d) below those of wild wheat for Kl-level (KC1 application rate of 186 kg·hm-2). For transgenic wheat high potassium absorption, available K is transported from non-rhizosphere to rhizosphere soil. Under K0 (no KC1), K1 and K2 (KC1 application rate of 805 kg·hm-2) treatments, biomass K use efficiencies (KUI-B) were 22.39%, 136.21% and 14.03%, respectively. Also while shoot K contents were 225.97%, 18.77% and 17.28%, root cation exchange capacities (CEC) were 55.19%, 33.16% and 30.44%, respectively. Rhizosphere soil catalase and urease activities of transgenic wheat were significantly higher than those of wild wheat at different K-levels. Thus transgenic technology improved root CEC and created good rhizosphere environment for efficient wheat potassium absorption and utilization.关键词
转基因小麦/土壤钾素/钾高效利用/土壤酶活性/阳离子交换量Key words
Transgenic wheat, Soil potassium, Potassium utilization, Soil enzyme activity, Cation exchange capacity分类
农业科技引用本文复制引用
阮丽,张佳宝,信秀丽,程宪国..转EdHP1(氢离子焦磷酸化酶)基因小麦的钾利用特征研究[J].中国生态农业学报,2012,20(8):1048-1053,6.基金项目
国家重点基础研究发展计划(973计划)项目(2011CB100500)、转基因生物新品种培育科技重大专项(2008ZX08002-005)和国家重点新技术推广项目(0841030000)资助 （973计划）
