生态学杂志2012，Vol.31Issue(8)：1911-1916,6.
孑遗植物水松不同年龄级种群遗传多样性的ISSR分析
Genetic diversity of different life-stage population of Glyptostrobus pensilis, an endangered plant in China: ISSR analysis
摘要
Abstract
Glyptostrobus pensilis is a rare and endangered species in China, but few studies have been made on the conservation genetics of this species. In this study, the G. pensilis population in Pingnan of Fujian Province, East China was divided into 3 life stages, i. e. , adult, juvenile, and seedling, according to the size class, and the genetic diversity and genetic differentiation of different life stage G. pensilis were investigated by using inter-simple sequence repeat ( ISSR) markers. Ten ISSR primers were used to amplify 72 individuals in the population, and 83 discernible DNA fragments were produced, of which, 32 were polymorphic loci (the percentage of polymorphic loci (P) = 38.55% ), suggesting the low level genetic diversity of the population. The difference of the genetic diversity among the life stages was evident. Adult had the highest P, Nei's gene index (He ) , and Shannon information index (I) , followed by juvenile, and seedling, suggesting that the genetic diversity among the life stages presented a declining trend. The analysis of molecular variance (AMOVA) showed that the genetic diversity might exist within and among the life stages, mainly within the life stages. Moreover, the genetic differential index (Gst) among the life stages was 0. 2872, while the gene flow (Nm) was 0. 6203. The genetic identity between seedlings and juveniles was the highest. Based on the analysis of population genetic structure and the community investigation, it would be necessary to establish a conserva-tion site in the optimal habitat of G. pensilis and to cross-transplant adult plants and seedlings mutually to enhance the gene flow. By this means, the genetic diversity resources of the species could be preserved to the greatest extent.关键词
水松/年龄级/遗传多样性/遗传分化/ISSRKey words
Glyptostrobus pensilis/ life stage/ genetic diversity/ genetic differentiation/ ISSR.分类
生物科学引用本文复制引用
吴则焰,刘金福,洪伟,潘东明,郑世群,何中声..孑遗植物水松不同年龄级种群遗传多样性的ISSR分析[J].生态学杂志,2012,31(8):1911-1916,6.基金项目
福建省自然科学基金项目(2009J01053)资助 （2009J01053）
