生态学杂志2012，Vol.31Issue(8)：2158-2162,5.
液质联用法测定干旱胁迫下红砂(Reaumuria soongorica)叶片F3H、DFR酶活性
Determination of Reaumuria soongorica leaf F3H and DFR activities under drought stress by using RP-HPLC-MS
摘要
Abstract
Flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR) are the key enzymes in flavonoids biosynthesis pathway, and to study their activities is a crucial step to explore the regulation of this pathway. In this paper, a RP-HPLC-MS determination method of F3 H and DFR activities was established to investigate the effects of drought stress on the activities of F3H and DFR in Reaumuria soongorica leaves. The calibration range of F3H substrate narin-genin and of DFR substrate dihydroquertin was 0.04-1. 20 μg (r = 0.998) and 0.08-2.40 μg (r = 0. 998) , respectively, suggesting that this method was convenient and accurate to determine the activities of F3H and DFR. By using this method, the F3H and DFR activities in R. soongorica leaves were measured, and the results showed that under drought stress, the F3H activity increased first and then dropped to the same level as the control, while the DFR activity increased all along with increasing drought time, indicating that drought stress had induction effect on the F3H and DFR activities in R. soongorica leaves.关键词
干旱胁迫/红砂/黄烷酮3-羟化酶/二氢黄酮醇4-还原酶/反相高效液相色谱-质谱法Key words
drought stress/ Reaumuria soongorica/ flavanone 3-hydroxylase (F3H) / dihydroflavonol 4-reductase (DFR)/ RP-HPLC-MS.分类
生物科学引用本文复制引用
刘美玲,刘玉冰,曹波..液质联用法测定干旱胁迫下红砂(Reaumuria soongorica)叶片F3H、DFR酶活性[J].生态学杂志,2012,31(8):2158-2162,5.基金项目
国家自然科学基金项目(30800122、31070358)资助. （30800122、31070358）
