生物加工过程2012，Vol.10Issue(4)：1-6,6.DOI:10.3969/j.issn.1672-3678.2012.04.001
响应面法优化青枯病生防菌Hrp-菌株的发酵条件
Optimization of fermentation conditons for Hrp-mutant of Ralstonia solanacearum as biocontrol agent of bacterial wilt using response surface methodology
摘要
Abstract
Optimization of fermentation conditions for Hrp mutant of Ralstonia solanacearum was conducted in flask. Temperature and shaking rate affecting the biomass of Hrp mutant were screened out by applying Plackett-Burman design. The path of steepest ascent was used to approach the optimal levels of the two important factors. The optimal levels were determined by the central composite design. The optimized fermentation conditions were as follows; inoculation time 10 h, fermentation temperature 20 ℃, initial pH 8. 75 , inoculation concentration 6. 25% , flask column 100 mL in 500 mL flask, and shaking rate 205 r/min. Under the optimal conditions, the bacterial biomass increased from 3. 30 X 1010 to 4. 42 X 1010 cfu/mL, an increase of 133. 9% .关键词
青枯病/发酵/Hrp-菌株/优化/响应面法Key words
bacterial wilt/ fermentation/ Hrp- strain/ optimization/ response surface methodology分类
农业科技引用本文复制引用
冉淦侨,田云龙,郭萍,刘雪,叶婧,张丽,朱昌雄..响应面法优化青枯病生防菌Hrp-菌株的发酵条件[J].生物加工过程,2012,10(4):1-6,6.基金项目
国家农业部"948"计划资助项目(2006-G54) （2006-G54）
陕西省青年人才培养专项基金资助项目(2010K-27) （2010K-27）
