陕西师范大学学报：自然科学版2012，Vol.40Issue(4)：70-75,6.
秦岭地区华中五味子天然居群ISSR遗传多样性分析
Analysis of genetic diversity of Schisandra sphenanthera Rehd. et Wils. natural populations in Qinling Mountain by ISSR marker
摘要
Abstract
The population genetic structure and genetic diversity of 90 individuals in three natural populations of Schisandra sphenanthera in Qinling Mountain were analysed based on ISSR molecular marker. Twelve inter-simple sequence repeat （ISSR） primers were used, 77 loci in which 65 were polymorphic and percentage of polymorphic loci was 85. 71 %. Shannon＇ s information index （I） and index of gene diversity （H） were 0. 507 and 0. 348 by square root method,0. 395 and 0. 382 by Bayesian Method, respectively. This shows that there was abundant genetic diversity in Schisandra sphenanthera. Genetic differentiation coefficients were between 0. 052 and 0. 106（φST =0. 106.FsT =0. 082.GsT =0. 066,θST=0. 052）, Gene flow （Nm） were between 2. 108 and 4. 558. The main genetic variation occurred within populations in Schisandra sphenanthera, the genetic differentiation among populations was very limited. The values of thegenetic differentiation coefficients from different definitions were significantly dissimilarity. Therefore, they should not be used for comparison directly. The biological characteristics of Schisandra sphenanthera, such as dioecy, wind-pollination or insect-pollination, wide-area distribution, were the main reasons for the formation of genetic diversity.关键词
华中五味子/遗传多样性/居群遗传结构/简单重复序列间区/秦岭Key words
Schisandra sphenanthera Rehd. et Wils./genetic diversity/population geneticstructure/inter-simple sequence repeat (ISSR)/Qinling Mountain分类
生物学引用本文复制引用
罗成,熊宇婷,顾蔚,王喆之..秦岭地区华中五味子天然居群ISSR遗传多样性分析[J].陕西师范大学学报：自然科学版,2012,40(4):70-75,6.基金项目
国家自然科学基金资助项目 （）
国家“十一五”科技支撑计划项目 （）
陕西师范大学研究生培养创新基金项目（2009CXS032）. （）
