山西医科大学学报2012，Vol.43Issue(7)：481-484,561,5.DOI:10.3969/J.ISSN.1007-6611.2012.07.001
EPO对大鼠脑缺血再灌注后脑水肿及MMP-9的影响
Effects of erythropoietin on brain edema and expression of matrix metalloproteinases-9 in rats after cerebral ischemia-reperfusion
何娅 1陈松盛 1王辉 1冯建军1
作者信息
- 1. 西安交通大学医学院第二附属医院老年病科,西安710004
- 折叠
摘要
Abstract
Objective To investigate the effects of erythropoietin( EPO) on the score of neurological deficits, brain edema and expression of matrix metalloproteinases-9 ( MMP-9) in rats after cerebral ischemia-reperfusion. Methods A total of 150 rats were randomly divided into sham operation group, ischemia group and EPO group. The model of focal brain ischemia-reperfusion was made by occluding the middle cerebral artery( MCA) for 2 h. The rats in ischemia group were intraperitoneally injected 2 ml saline 10 min before reperfusion, and rhEPO (3 000 U/kg) +2 ml saline in EPO group. The score of neurological deficits was determined at 6 h, 12 h,48 h, 72 h ,7 d and 10 d after reperfusion. The water content in brain tissues was measured with Elliot formula and the expression of MMP-9 was examined with immunohistochemical stain. Results hi ischemia group,the score of neurological deficits increased at 6 h,peaked at 48 h.and began to decrease at 72 h;the water content increased at 6 h.and peaked at 24 -48 h;the positive expression of MMP-9 was observed at 6 h after brain ischemia-reperfusion,increased at 12 h,peaked at 48 h.and gradually decreased at 72 h,7 d and 10 d. Compared with ischemia group,the score of neurological deficits,the water content in brain tissues and MMP-9 positive cells decreased significantly in EPO group(P<0.05). Conclusion The results suggest that EPO may protect against cerebral iscbemia-reperfusion injury,which is related to the decrease of expression of MMP-9.关键词
脑缺血再灌注/神经功能缺损评分/脑水肿/EPO/MMP-9Key words
cerebral ischemia and reperfusion/ brain edema/ the score of neurological deficits/ erythropoietin/ matrix分类
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何娅,陈松盛,王辉,冯建军..EPO对大鼠脑缺血再灌注后脑水肿及MMP-9的影响[J].山西医科大学学报,2012,43(7):481-484,561,5.
