现代检验医学杂志2012，Vol.27Issue(2)：11-14,4.DOI:10.3969/j.issn.1671-7414.2012.02.004
CdTe量子点荧光探针在生物分析中的应用
Application of Quantum Dots as Fluoresent Probes in Biological Analysis
摘要
Abstract
Objective To investigate the application of CdTe quantum dots as fluoresent probes in Biological analysis. Methods Prepared CdTe quantum dots in aqueous phase and coating with MPA. Exosyndrome QDs with TEM and Fluorspectro-photometer. Binded QDs to avidin, purified the connective and prepared it as flurescent probe. LSCM was used to observe the QDs fluorescent probe label expression of MHCⅡ antigen on PMΦ and used MTT to observe the biocompatibility of CdTe quantum dots coated with MPA using the H22 cells as target cells. Results The particle diameter of CdTe quantum dots prepared in aqueous phase was well-distributed and it had good photology performance. QDs had little cytotoxicity within some concentration range. Conclusion CdTe QDs has good biocompatibility. It's not only fit for immunofluorescence label of fixed histiocyte but also can satisfy the experiment desire of direct or dynamic label of live cells.关键词
量子点/荧光探针/生物分析/H22肝癌细胞Key words
quantum dots/fluoresent probes/biological analysis/ H22 cells分类
生物科学引用本文复制引用
胡兵,韩崇旭..CdTe量子点荧光探针在生物分析中的应用[J].现代检验医学杂志,2012,27(2):11-14,4.基金项目
江苏省自然科学基金(BK2007072). （BK2007072）
