现代检验医学杂志2012，Vol.27Issue(2)：60-62,65,4.DOI:10.3969/j.issn.1671-7414.2012.02.021
荧光原位杂交法(FISH)快速检测胎儿染色体异常及临床应用评价
Rapid Detection of Chromosomal Abnormalities in Clinical Amniotic Fluid Samples by Fluorescence in Situ Hybridization
潘小平 1赖金国 1许伟华 1钟小青 1蔡高涛 1李素梅1
作者信息
- 1. 广州市花都区人民医院检验科,广州 510800
- 折叠
摘要
Abstract
Objective To establish a rapid method in detecting chromosomal abnormalities of fetus in clinical amniotic fluid samples to assist prenatal diagnosis. Methods Using fluorescent in situ hybridization (FISH) with different fluoresceins labelled the five chromosomes that could easily induce hereditary disease in clinical. Chromosomal abnormalities were rapidly detected and identified in 145 clinical amniotic fluid samples and compared with the conventional karyotype analysis. Then estimated the results of FISH method. Results The result of this method established in the research was stable. Two triso-my 21 samples were successfully detected positively in all 145 clinical amniotic fluid samples. Compared with the karyotype analysis,the sensitivity and the specificity of FISH were 66. 67% (2/3) and 100. 00% (142/142) respectively. The positive predictive value was 100. 00% (2/2) and the negative predictive value was 99. 30% (142/143) respectively. Conclusion Fluorescent in situ hybridization is a rapid,specific,highly sensitive technique for the detection chromosomal abnormalities of fetus and could rapidly diagnosis for pregnant women.关键词
原位杂交/荧光/染色体异常/胎儿/快速检测Key words
in situ hybridization /fluorescence /chromosomal abnormalities/ fetus /rapid identification分类
医药卫生引用本文复制引用
潘小平,赖金国,许伟华,钟小青,蔡高涛,李素梅..荧光原位杂交法(FISH)快速检测胎儿染色体异常及临床应用评价[J].现代检验医学杂志,2012,27(2):60-62,65,4.
